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Recombinant Human Ras Homolog Gene Family Member B

Source : Escherichia Coli. RHOB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193 a.a.) and having a molecular mass of 23.9kDa (Molecular weight on SDS-PAGE will appear higher) .RHOB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Rho-related GTP-binding protein RhoB (RhoB) belongs to the Rho GTP-binding protein family. RhoB facilitates apoptosis in neoplastically transformed cells after DNA damage. RhoB has a negative role in tumorigenesis since deletion causes tumor formation.

Product Specifications

Product Name Alternative

Rho-related GTP-binding protein RhoB||Rho cDNA clone 6||h6||RHOB||ARH6||ARHB||RHOH6||MST081||MSTP081.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

RHOB protein solution (0.25mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol and 0.2mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC.

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