Recombinant Human RAP2A, Member RAS Oncogene Family

Source : Escherichia Coli. RAP2A Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 200 amino acids (1-180 a.a.) and having a molecular mass of 22.4kDa. The RAP2A is purified by proprietary chromatographic techniques. RAP2A belongs to the RAS oncogene family and a Ras-like small G protein. The RAP2A protein shares 60% identity with Rap1A and exhibits a carboxy-terminal CAAX motif and 2 upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap1A and Rap1B, overexpression of RAP2A doesn't obstruct the Ras signaling pathway.

Product Specifications

Product Name Alternative

Ras-related protein Rap-2a||RbBP-30||RAP2A||KREV||RAP2||K-REV.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

The RAP2A solution (1 mg/mL) 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.

Storage Conditions

RAP2A should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI IRVKRYEKVP VILVGNKVDL ESEREVSSSE GRALAEEWGC PFMETSAKSK TMVDELFAEI VRQMNYAAQP DKDDPCCSAC.