Recombinant Human Proteasome Assembly Chaperone 4

Source : E.coli. PSMG4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 143 amino acids (1-123 a.a.) and having a molecular mass of 15.9kDa.PSMG4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. PSMG4 is a chaperone protein that enhances assembly of the 20S proteasome. PSMG4 cooperates with alpha and beta subunits of the 20S proteasome and with PSMG3 but disconnects when the POMP is recruited before the formation of half-proteasomes.

Product Specifications

Product Name Alternative

Proteasome (prosome||macropain) assembly chaperone 4||C6orf86||hPAC4||chromosome 6 open reading frame 86||bA506K6.2.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

PSMG4 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MEGLVVAAGG DVSLHNFSAR LWEQLVHFHV MRLTDSLFLW VGATPHLRNL AVAMCSRYDS IPVSTSLLGD TSDTTSTGLA QRLARKTNKQ VFVSYNLQNT DSNFALLVEN RIKEEMEAFP EKF