Recombinant Human Protein Phosphatase 1, Regulatory Subunit 3B

Source : Escherichia Coli. PPP1R3B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-285) and having a molecular mass of 35.1 kDa.PPP1R3B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Protein Phosphatase 1, Regulatory Subunit 3B (PPP1R3B) is the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. PPP1R3B, which is expressed both in liver and in skeletal muscle tissue, regulates glycogen synthesis in these tissues. PPP1R3B is involved in type 2 diabetes and maturity-onset diabetes of the young.

Product Specifications

Product Name Alternative

Protein phosphatase 1 regulatory subunit 3B||Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL||Protein phosphatase 1 regulatory subunit 4||PP1 subunit R4||Protein phosphatase 1 subunit GL||PTG||PPP1R4||PPP1R3B||Protein Phosphata

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

The PPP1R3B solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMMAVDIE YRYNCMAPSL RQERFAFKIS PKPSKPLRPC IQLSSKNEAS GMVAPAVQEK KVKKRVSFAD NQGLALTMVK VFSEFDDPLD MPFNITELLD NIVSLTTAES ESFVLDFSQP SADYLDFRNR LQADHVCLEN CVLKDKAIAG TVKVQNLAFE KTVKIRMTFD TWKSYTDFPC QYVKDTYAGS DRDTFSFDIS LPEKIQSYER MEFAVYYECN GQTYWDSNRG KNYRIIRAEL KSTQGMTKPH SGPDLGISFD QFGSPRCSYG LFPEWPSYLG YEKLGPYY.