Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide J

Source : Escherichia Coli. POLR2J Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117 a.a) and having a molecular mass of 15.7kDa.POLR2J is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Polymerase (RNA) II (DNA directed) Polypeptide J (POLR2J) is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The POLR2J protein occurs as a heterodimer with another polymerase subunit; together they develop a core subassembly unit of the polymerase. POLR2J is ubiquitously expressed, having a high expression in the heart and skeletal muscle.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerase II subunit RPB11-a||RNA polymerase II subunit B11-a||RPB11a||DNA-directed RNA polymerase II subunit J-1||RNA polymerase II 13.3 kDa subunit||POLR2J||POLR2J1||RPB11||RPB11m||hRPB14.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

POLR2J protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE.