Recombinant Human Polymerase (RNA) II (DNA directed) Polypeptide C

Source : Escherichia Coli. POLR2C Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-275a.a) and having a molecular mass of 33.8kDa. POLR2C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Polymerase (RNA) II (DNA directed) Polypeptide C (POLR2C) is the 3rd largest subunit of RNA polymerase II. The product of POLR2C consists of a cysteine rich area and exists as a heterodimer with an additional polymerase subunit, POLR2J. Those 2 subunits shape a core subassembly unit of the polymerase.

Product Specifications

Product Name Alternative

HRPB33||hsRPB3||RPB3||RPB31||DNA-directed RNA polymerase II subunit RPB3||RNA polymerase II subunit 3||RNA polymerase II subunit B3||DNA-directed RNA polymerase II 33 kDa polypeptide||RPB33||DNA-directed RNA polymerase II subunit C||POLR2C.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

POLR2C protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol 1mM DTT and 0.1M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMPYANQP TVRITELTDE NVKFIIENTD LAVANSIRRV FIAEVPIIAI DWVQIDANSS VLHDEFIAHR LGLIPLISDD IVDKLQYSRD CTCEEFCPEC SVEFTLDVRC NEDQTRHVTS RDLISNSPRV IPVTSRNRDN DPNDYVEQDD ILIVKLRKGQ ELRLRAYAKK GFGKEHAKWN PTAGVAFEYD PDNALRHTVY PKPEEWPKSE YSELDEDESQ APYDPNGKPE RFYYNVESCG SLRPETIVLS ALSGLKKKLS DLQTQLSHEI QSDVLTIN.