Recombinant Human Penta-EF-Hand Domain Containing 1

Source : Escherichia Coli. PEF1 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 320 amino acids (1-284 a.a.) and having a molecular mass of 34.5kDa. The PEF1 is purified by proprietary chromatographic techniques. Penta-EF-hand domain containing 1 (PEF1) is a member of the penta-EF-hand protein family, which shares similarities to the apoptosis linked gene ALG-2. PEF1 forms heterodimers with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca (2+) . Furthermore, PEF1 proteins bind calcium and participate in a various calcium-dependent processes in vertebrates.

Product Specifications

Product Name Alternative

Peflin||PEF protein with a long N-terminal hydrophobic domain||Penta-EF hand domain-containing protein 1||PEF1||ABP32||PEF1A.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

The PEF1 solution (0.25 mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50% glycerol and 0.2M NaCl.

Storage Conditions

PEF1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASY PYRQGCPGAA GQAPGAPPGSYYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGAAPGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALVNCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYTELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAVQGNIRLSFED FVTMTASRML.