Recombinant Human PEST proteolytic Signal Containing Nuclear Protein

Source : Escherichia Coli. PCNP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 202 amino acids (1-178 a.a.) and having a molecular mass of 21.5kDa.PCNP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. PEST proteolytic signal containing nuclear protein (PCNP) is a nuclear protein involved in cell cycle regulation and tumorigenesis. The PCNP protein is ubiquitinated post-translationally by NIRF (an ubiquitin ligase) . The PCNP exists as 3 isoforms produced by alternative splicing events.

Product Specifications

Product Name Alternative

PEST proteolytic signal-containing nuclear protein||PCNP||PEST-containing nuclear protein.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

PCNP protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN.