Recombinant Human Non-Metastatic Cells 4

Source : Escherichia Coli. NME4 Human Recombinant fused with a 210 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa. The NME4 is purified by proprietary chromatographic techniques. NME4 is a member of the NDK family. NME4 are ubiquitous enzymes which catalyze transfer of gamma-phosphates, using a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family that include NME4. NME4 has a key part in the synthesis of nucleoside triphosphates other than ATP.

Product Specifications

Product Name Alternative

Non-metastatic cells 4||nm23-H4||NM23H4||Nucleoside diphosphate kinase D||NDK||NDPKD||NDP kinase mitochondrial||EC 2.7.4.6||NDPK-D.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The NME4 solution (0.5mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 40% glycerol and 0.2M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA.