Recombinant Human Neuronal Calcium Sensor 1

Source : Escherichia Coli. NCS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 198 amino acids (1-190 a.a.) and having a molecular mass of 22.9 kDa. The NCS1 is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques. NCS1 is part of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed primarily in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. NCS1 is related with secretory granules and modulates synaptic transmission and synaptic plasticity. NCS1 regulates GRK1 and substitutes for calmodulin. NCS1 stimulates PI4KB kinase activity and participates in long-term synaptic plasticity through its interaction with PICK1. NCS1 takes part in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Product Specifications

Product Name Alternative

Frequenin homolog||FLUP||FREQ||Neuronal calcium sensor 1||Frequenin-like protein||Frequenin-like ubiquitous protein||NCS-1||NCS1||DKFZp761L1223.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The NCS1 solution contains 20mM Tris-HCl pH-8, 1mM DTT, and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH.