Recombinant Human Neuroblastoma 1

Source : E.coli. NBL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids (18-181 a.a) and having a molecular mass of 20kDa. NBL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Neuroblastoma suppressor of tumorigenicity 1 (NBL1) is a part of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in several signaling molecules. NBL1 is a is a tumor suppressor of neuroblastoma and takes part in preventing cells from entering the final stage (G1/S) of the transformation process. NBL1 is produced in small neurons of the dorsal root ganglion. The expression of NBL1 is triggered by MATH-1.

Product Specifications

Product Name Alternative

D1S1733E||DAN||DAND1||NB||NO3||Neuroblastoma suppressor of tumorigenicity 1||DAN domain family member 1||Zinc finger protein DAN||NBL1.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

NBL1 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSAPPPINK LALFPDKSAW CEAKNITQIV GHSGCEAKSI QNRACLGQCF SYSVPNTFPQ STESLVHCDS CMPAQSMWEI VTLECPGHEE VPRVDKLVEK ILHCSCQACG KEPSHEGLSV YVQGEDGPGS QPGTHPHPHP HPHPGGQTPE PEDPPGAPHT EEEGAED.