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Recombinant Human Myoglobin, His Tag

Source : Escherichia Coli. MB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154a.a.) and having a molecular mass of 19.3kDa.MB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Myoglobin, mitochondrial precursor is a cytosolic oxygen-binding protein in charge of the storage and diffusion of oxygen within myocytes. The largest expression of MB is in the skeletal and cardiac muscle. MB operates in various functions in muscular oxygen supply, such as oxygen storage, facilitated diffusion, and myoglobin-mediated oxidative phosphorylation.

Product Specifications

Product Name Alternative

PVALB||MGC13548.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The MB protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 20% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG

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