Recombinant Human Myosin Light Chain 12B

Source : E.coli. MYL12B Human Recombinant produced in E. coli is a single polypeptide chain containing 196 amino acids (1-172) and having a molecular mass of 22.3kDa.MYL12B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Myosin is composed of 2 nonphosphorylatable alkali light chains, 2 heavy chains and 2 phosphorylatable regulatory light chains. MYL12B is a hexameric ATPase cellular motor protein which controls contraction in smooth muscle and non-muscle cells through phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of myosin regulatory light chains catalyzed by MLCK in the presence of calcium and calmodulin, rises Actin-activated myosin ATPase activity, thus regulating contractile activity.

Product Specifications

Product Name Alternative

Myosin light chain 12B regulatory||Myosin regulatory light chain 2-B smooth muscle isoform||Myosin regulatory light chain 20 kDa||Myosin regulatory light chain MRLC2||MYLC2B||MRLC2||MLC-2A||MLC-B||SHUJUN-1 MLC20.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The MYL12B solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 2mM DTT and 40% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD