Recombinant Human Metallothionein 3

Source : Escherichia Coli. MT3 Human Recombinant produced in E. coli is a single polypeptide chain containing 91 amino acids (1-68) and having a molecular mass of 9.3kDa (molecular size on SDS-PAGE will appear higher) .MT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Metallothionein 3 (MT3) contains 3 zinc and 3 copper atoms per polypeptide chain and a minor amount of cadmium. MT3 inhibits survival and neurite formation of cortical neurons in vitro. MT3 also binds heavy metals.

Product Specifications

Product Name Alternative

GIF||GIFB||GRIF||ZnMT3||Metallothionein-3||MT-3||Growth inhibitory factor||Metallothionein-III||MT-III||MT3.

Purification

Greater than 85% as determined by SDS-PAGE.

Components

The MT3 solution (0.5mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.