Recombinant Human Linker for Activation of T Cells

Source : E.coli. LAT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (28-233) and having a molecular mass of 24.4 kDa (Molecular weight on SDS-PAGE will appear higher) .LAT is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. LAT is a vital membrane protein that is phosphorylated by ZAP-70/Syk protein tyrosine kinases succeeding activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes.

Product Specifications

Product Name Alternative

Linker for activation of T cells||LAT1||36 kDa phospho-tyrosine adapter protein||p36-38||pp36||linker for activation of T cells transmembrane adaptor||linker for activation of T-cells family member 1.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The LAT solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0) and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MHCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN