Recombinant Human Cytokeratin 18

Source : Escherichia Coli. Cytokeratin 18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a calculated molecular mass of 48,201 Dalton, showing a 45kDa band on SDS-page, pI-5.7.The KRT18 is purified by proprietary chromatographic techniques. KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Product Specifications

Product Name Alternative

Keratin type I cytoskeletal 18||Cytokeratin-18||CK-18||Keratin-18||K18||KRT18, CYK18, Cell proliferation-inducing gene 46 protein.

Purification

Greater than 95.0% as determined by analysis by SDS-PAGE.

Components

The protein (1mg/mL) was lyophilized from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM EDTA, 2mM DTT and 10mM methylammonium chloride.

Storage Conditions

Lyophilized CK-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CK-18 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized CK-18 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.