Recombinant Human Iron-sulfur Cluster Scaffold Homolog

Source : Escherichia Coli. ISCU Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids (35-167 a.a.) and having a molecular mass of 16.7kDa. The ISCU is purified by proprietary chromatographic techniques. Iron-sulfur cluster assembly enzyme (ISCU) belongs to the nifU family. Iron-sulfur (Fe-S) clusters are required for several mitochondrial enzymes and other subcellular compartment proteins. ISCU interacts with ISCS (a cysteine desulfurase) to sequester inorganic sulfur for Fe-S cluster assembly. The ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly occurs in multiple subcellular compartments in mammalian cells.

Product Specifications

Product Name Alternative

Iron-sulfur cluster assembly enzyme ISCU mitochondrial||NifU-like N-terminal domain-containing protein||NifU-like protein||ISCU||NIFUN||HML||ISU2||NIFU||hnifU||MGC74517||2310020H20Rik.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The ISCU solution (0.5 mg/mL) 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl.

Storage Conditions

ISCU should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK.