IMPAD1 Recombinant Protein

Source : Escherichia Coli. IMPAD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (34-359 a.a) and having a molecular mass of 37.6kDa.IMPAD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Inositol monophosphatase 3 (IMPAD1) belongs to the inositol monophosphatase family. IMPAD1 is restricted to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP) . IMPAD1 gene mutations cause the GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of the IMPAD1 gene is located on the long arm of chromosome 1.

Product Specifications

Product Name Alternative

Inositol monophosphatase 3||IMP 3||IMPase 3||EC 3.1.3.25||EC 3.1.3.7||Golgi 3-prime phosphoadenosine 5-prime phosphate 3-prime phosphatase||Golgi-resident PAP phosphatase||gPAPP||Inositol monophosphatase domain-containing protein 1||Inositol-1 (or 4) -

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

IMPAD1 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 2M Urea and 20% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.