HMGN3 Recombinant Protein

Source : Escherichia Coli. HMGN3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 100 amino acids (1-77 a.a) and having a molecular mass of 10.8 kDa. HMGN3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. High Mobility Group Nucleosomal Binding Domain 3 (HMGN3), binds thyroid hormone receptor beta, however it occurs only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors which regulate expression of a variety of particular target genes. HMGN3 is considered to reduce the compactness of the chromatin fiber in nucleosomes, in that way enhancing transcription from chromatin templates.

Product Specifications

Product Name Alternative

High Mobility Group Nucleosomal Binding Domain 3||TRIP7, TR-Interacting Protein 7||High Mobility Group Nucleosome-Binding Domain-Containing Protein 3||Thyroid Hormone Receptor Interacting Protein 7||Thyroid Receptor-Interacting Protein 7||Thyroid Hor

Purification

Greater than 95% as determined by SDS-PAGE.

Components

HMGN3 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH7.0) and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.