HLA-DRB1 Recombinant Protein

Source : Escherichia Coli. HLA-DRB1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (30-227 a.a) and having a molecular mass of 25.2kDa.HLA-DRB1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Major Histocompatibility Complex Class II DR Beta 1 also known as HLA-DRB1 ia a member of the HLA class II beta chain paralogs. Molecule class II is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. HLA-DRB1 takes an essential part in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . Furthermore, the beta chain is approximately 26- 28 kDa. It is encoded by 6 exons. While exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail.

Product Specifications

Product Name Alternative

DRB1||HLA DRB1||HLA-DR1B||HLA-DRB1||MHC class II antigen DRB1 16||DR-16||DR16||Human Leucocyte AntigenDRB1||MHC Class IIHLA-DR-Beta Cell Surface Glycoprotein||MHC Class IIHLA-DRw10-Beta.

Purification

"Greaterthan 85% as determined by SDS-PAGE."

Components

HLA-DRB1 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please avoid freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK