GCSH Recombinant Protein

Source : E.coli. GCSH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 149 amino acids (48-173) and having a molecular mass of 16.4 kDa.GCSH is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. There are four mitochondrial proteins composing the enzyme system for cleavage of glycine (glycine cleavage system) : P protein, H protein, T protein, and L protein. GCSH is the H protein. GCSH transfers the methylamine group of glycine from the P protein to the T protein. Mutations in this gene results in nonketotic hyperglycinemia (NKH) .

Product Specifications

Product Name Alternative

Glycine cleavage system protein H (aminomethyl carrier) ||NKH||GCE||Lipoic acid-containing protein||Mitochondrial glycine cleavage system H-protein.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

The GCSH solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.15M NaCl and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE

GCSH Recombinant Protein

Product Specifications

Product Name Alternative

Purification

Components

Storage Conditions

Amino Acids

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