FNDC5 Yeast Recombinant Protein

Source : Yeast. Fibronectin Type III Domain Containing 5 Human Recombinant produced in yeast is a glycosylated, polypeptide chain containing 110 amino acids and having a molecular mass of 20-25 kDa.FNDC5 is purified by proprietary chromatographic techniques. Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT 'browning'. r-Irisin can reduce body weight and cause induction of brown adipocyte in vivo, including a capacity for thermogenic energy expenditure mediated by uncoupling protein 1 (UCP-1) . Irisin mature protein is comprised of 110 amino acid residues and two potential glycosylation sites. High levels of Irisin can be found in the heart whereas Very low expression is found in the colon, pancreas and spleen, if any.

Product Specifications

Product Name Alternative

Fibronectin type III domain-containing protein 5||Fibronectin type III repeat-containing protein 2||Irisin||FRCP2||FNDC5.

Purification

Greater than 98.0% as determined by SDS-PAGE.

Components

The FNDC5 was lyophilized from 0.45μm filtered solution in PBS.

Storage Conditions

Lyophilized Fibronectin Type III Domain Containing 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FNDC5 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the lyophilized Fibronectin Type III Domain Containing 5 in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The biological activity of recombinant human irisin was measured by the ability to induce UCP-1 expression of adipocytes. The specific activity is 10ng/ml.

Amino Acids

S_P__S__A__P__V__N__V__T__V__R__H__L__K__A__N__S__A__V__V__S_ W__D__V__L__E__D__E__V__V__I__G__F__A__I__S__Q__Q__K__K__D _V__R__M__L__R__F__I__Q__E__V__N__T__T__T__R__S__C__A__L__W_ _ D__L__E__E__D__T__E__Y__I__V__H__V__Q__A__I__S__I__Q__G__Q_ S__P__A__S__E__P__V__L__F__K__T__P__R__E__A__E__K__M__A__S_ K__N__K__D__E__V__T__M__K__E.