ETFB Recombinant Protein

Source : Escherichia Coli. ETFB Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 275 amino acids (1-255a.a.) and having a molecular mass of 30.0kDa. The ETFB is purified by proprietary chromatographic techniques. ETF is a heterodimer composed of alpha and beta subunit. ETFB protein is electron-transfer-flavoprotein, beta polypeptide that transports electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase.

Product Specifications

Product Name Alternative

Electron-transfer-flavoprotein beta polypeptide||MADD||beta-ETF.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The ETFB solution (0.25mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 40% glycerol and 0.1M NaCl.

Storage Conditions

ETFB should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MAELRVLVAV KRVIDYAVKI RVKPDRTGVV TDGVKHSMNP FCEIAVEEAV RLKEKKLVKE VIAVSCGPAQ CQETIRTALA MGADRGIHVE VPPAEAERLG PLQVARVLAK LAEKEKVDLV LLGKQAIDDD CNQTGQMTAG FLDWPQGTFA SQVTLEGDKLKVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK AKKKKIEVIK PGDLGVDLTS KLSVISVEDP PQRTAGVKVE TTEDLVAKLK EIGRI.