COX5A Recombinant Protein

Source : Escherichia Coli. COX5A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (42-150 a.a) and having a molecular mass of 14.9kDa.COX5A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Cytochrome C Oxidase Subunit Va (COX5A) is a member of the cytochrome c oxidase subunit 5A family. COX (Cytochrome C Oxidase), which is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced Cytochrome C to oxygen. The COX component is a heteromeric complex comprised of three catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits serve in electron transfer, and the nuclear-encoded subunits act in the regulation and assembly of the complex. COX5A is the heme A-containing chain of Cytochrome C Oxidase, which is the terminal oxidase in mitochondrial electron transport.

Product Specifications

Product Name Alternative

Cytochrome c oxidase subunit 5A||mitochondrial||Cytochrome c oxidase polypeptide Va||COX5A||VA||COX||COX-VA.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

COX5A protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 2mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.