CENPM Recombinant Protein

Source : Escherichia Coli. CENPM Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-180) and having a molecular mass of 22.0kDa.CENPM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.

Product Specifications

Product Name Alternative

Centromere Protein M||Interphase Centromere Complex Protein 39||Chromosome 22 Open Reading Frame 18||Proliferation Associated Nuclear Element 1||bK250D10.2||CENP-M||ICEN39||C22orf18||PANE1.

Purification

Greater than 85% as determined by SDS-PAGE.

Components

The CENPM solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMSVLRPL DKLPGLNTAT ILLVGTEDAL LQQLADSMLK EDCASELKVH LAKSLPLPSS VNRPRIDLIV FVVNLHSKYS LQNTEESLRH VDASFFLGKV CFLATGAGRE SHCSIHRHTV VKLAHTYQSP LLYCDLEVEG FRATMAQRLV RVLQICAGHV PGVSALNLLS LLRSSEGPSL EDL