MPD-L1 Recombinant Protein

Source : E.coli. CD274 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (19-239 a.a) and having a molecular mass of 27.6kDa (Molecular size on SDS-PAGE will appear higher) .CD274 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. CD274 takes part in the costimulatory signal, vital for T-cell proliferation and creation of IL10 and IFNG, in a PDCD1-independent and an IL2-dependent manner. CD274 collaboration with PDCD1 inhibits cytokine production and T-cell proliferation.

Product Specifications

Product Name Alternative

Programmed cell death 1 ligand 1||PD-L1||PDCD1 ligand 1||Programmed death ligand 1||B7 homolog 1||B7-H1||CD274||B7h1||Pdcd1l1||Pdcd1lg1||Pdl1||A530045L16Rik.

Purification

Greater than 85% as determined by SDS-PAGE.

Components

CD274 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.15M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSHMFTITA PKDLYVVEYG SNVTMECRFP VERELDLLAL VVYWEKEDEQ VIQFVAGEED LKPQHSNFRG RASLPKDQLL KGNAALQITD VKLQDAGVYC CIISYGGADY KRITLKVNAP YRKINQRISV DPATSEHELI CQAEGYPEAE VIWTNSDHQPVSGKRSVTTS RTEGMLLNVT SSLRVNATAN DVFYCTFWRS QPGQNHTAEL IIPELPATHP PQNRTH.