C9ORF95 Recombinant Protein

Source : Escherichia Coli. C9ORF95 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 222 amino acids (1-199 a.a) and having a molecular mass of 25.6kDa.C9ORF95 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Chromosome 9 Open Reading Frame 95 (C9ORF95/NRK1) is a member of the uridine kinase family. NAD+ is vital for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in countless reactions, including those which delay aging in experimental systems. Nicotinic acid and nicotinamide are defined as the vitamin precursors of NAD+. C9ORF95 catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN) . C9ORF95 also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine.

Product Specifications

Product Name Alternative

Nicotinamide riboside kinase 1||NRK 1||NmR-K 1||Nicotinic acid riboside kinase 1||Ribosylnicotinamide kinase 1||RNK 1||Ribosylnicotinic acid kinase 1||NMRK1||C9orf95||NRK1||bA235O14.2||RP11-235O14.2.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

C9ORF95 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT and 200mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMKTFIIG ISGVTNSGKT TLAKNLQKHL PNCSVISQDD FFKPESEIET DKNGFLQYDV LEALNMEKMM SAISCWMESA RHSVVSTDQE SAEEIPILII EGFLLFNYKP LDTIWNRSYF LTIPYEECKR RRSTRVYQPP DSPGYFDGHV WPMYLKYRQE MQDITWEVVY LDGTKSEEDL FLQVYEDLIQ ELAKQKCLQV TA.