BUD31 Recombinant Protein

Source : Escherichia Coli. BUD31 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 19.4kDa.BUD31 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. BUD31 is a member of the BUD31 (G10) family. BUD31 is a nuclear protein which contains a putative nuclear translocation sequence, an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a putative Zinc-finger structure. Based on the structure of BUD31 it is suggested that BUD31 might be a nuclear regulator of transcription.

Product Specifications

Product Name Alternative

BUD31 homolog (S. cerevisiae) ||Cwc14||EDG-2||EDG2||fSAP17||G10||YCR063W||Protein G10 homolog.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

BUD31 protein solution (0.25mg/mL) contains 20mM Tris-HCl buffer, (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMPKVKRS RKAPPDGWEL IEPTLDELDQ KMREAETEPH EGKRKVESLW PIFRIHHQKT RYIFDLFYKR KAISRELYEY CIKEGYADKN LIAKWKKQGY ENLCCLRCIQ TRDTNFGTNC ICRVPKSKLE VGRIIECTHC GCRGCSG.