ATP1B3 Recombinant Protein

Source : Escherichia Coli. ATP1B3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (57-279) and having a molecular mass of 27.4 kDa.ATP1B3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. ATPaseTransporting Beta 3 (ATP1B3) is a member of the X (+) /potassium ATPases subunit beta family. ATP1B3 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit controls, by way of assembly of alpha/beta heterodimers, the quantity of sodium pumps transported to the plasma membrane. The precise role of the beta-3 subunit is unknown.

Product Specifications

Product Name Alternative

ATPase Na+/K+ Transporting Beta 3 Polypeptide||Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic) ||Sodium/Potassium-Transporting ATPase Subunit Beta-3||Sodium/Potassium-Dependent ATPase Subunit Beta-3||Sodium Pump Subunit Beta-3||ATPB-3||Sodium/P

Purification

Greater than 80.0% as determined by SDS-PAGE.

Components

The ATP1B3 solution (0.25mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.15M NaCl and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA.