ANXA4 Recombinant Protein

Source : Escherichia Coli. ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa. ANXA4 is purified by proprietary chromatographic techniques. ANXA4 is part of the annexin family of calcium-dependent phospholipid binding proteins. ANXA4 has 45%-59% identity with other proteins of its family and shares a related size and exon-intron organization. ANXA4 interacts with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is exclusively expressed in epithelial cells. ANXA4 is a Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Product Specifications

Product Name Alternative

ANX4||ANXA4||Annexin-IV||Lipocortin-4||Lipocortin-IV||Annexin-4||Annexin A4||Endonexin I||Chromobindin-4||Protein II||P32.5||Placental anticoagulant protein II||PAP-II||PP4-X||35-beta calcimedin||Carbohydrate-binding protein p33/p41||PIG28||ZAP36

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The ANXA4 protein solution contains 20mM Tris-HCl, pH-8 and 20% glycerol, 0.2M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles.

Amino Acids

MAMATKGGTV KAASGFNAME DAQTLRKAMK GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ VIVGMMTPTV LYDVQELRRA MKGAGTDEGC LIEILASRTP EEIRRISQTY QQQYGRSLED DIRSDTSFMF QRVLVSLSAG GRDEGNYLDD ALVRQDAQDL YEAGEKKWGT DEVKFLTVLC SRNRNHLLHV FDEYKRISQK DIEQSIKSET SGSFEDALLA IVKCMRNKSA YFAEKLYKSM KGLGTDDNTL IRVMVSRAEI DMLDIRAHFK RLYGKSLYSF IKGDTSGDYR KVLLVLCGGD D.