SKP1 Recombinant Protein

Source : Escherichia Coli. Recombinant Human SKP1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (1-160 a.a.) and having a molecular mass of 18kDa.SKP1 is purified by proprietary chromatographic techniques. SKP1 is a F-box enzyme which functions as a substrate recognition component of the SCF ubiquitin ligase complex which controls the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SKP1 binds to proteins containing an F-box motif, such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis. SKP1 takes part in the control of beta-catenin levels and the activity of beta-catenin dependent TCF transcription factors. SKP1 serves as an adapter that links the F-box protein to CUL1 in the SCF complex.

Product Specifications

Product Name Alternative

SKP-1||EMC19||MGC34403||OCP-II||OCP2||p19A||SKP1A||TCEB1L||S-phase kinase-associated protein 1||Cyclin-A/CDK2-associated protein p19||p19skp1||RNA polymerase II elongation factor-like protein||Organ of Corti protein 2||OCP-2||Organ of Corti protein I

Purification

Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The SKP1 protein solution contains 20mM Tris-HCl, pH-8, 10% glycerol and 50mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MPSIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV NAAILKKVIQ WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL FELILAANYL DIKGLLDVTC KTVANMIKGK TPEEIRKTFN IKNDFTEEEE AQVGSTQFCL.