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PI3KyD946GST Recombinant Protein

Source : Sf9 insect cells. The PI3KD946A protein is a catalytically inactive mutant of PI3K with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3K kinase activity studies. Recombinant full length PI3KgDA mutant (126.3 kDa without Tag) carries an N-terminal GST-Tag which facilitates the protein's application in typical GST pull-down assays.

Product Specifications

Product Name Alternative

Phosphoinositide 3-kinase p110?||PI3K?D946.

Purification

Greater than 95.0% as determined by SDS Page.

Components

0.4mg/mL solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.

Storage Conditions

PI3Kb although stable at 4°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.
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