CDKN3 Recombinant Protein

Source : Escherichia Coli. CDKN3 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 232 amino acids (1-212 a.a.) and having a molecular mass of 25.9kDa. The CDKN3 is purified by proprietary chromatographic techniques. Cyclin-dependent kinase inhibitor 3 (CDKN3) is a member of the dual specificity protein phosphatase family. CDKN3 was identified as a cyclin-dependent kinase inhibitor, and was shown to interact with, and dephosphorylate CDK2 kinase, consequently prevent the activation of CDK2 kinase. In addition CDKN3 is important in cell cycle regulation. The CDKN3 gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 3||CDK2-associated dual-specificity phosphatase||Cyclin-dependent kinase interactor 1||Cyclin-dependent kinase-interacting protein 2||Kinase-associated phosphatase||KAP||CDI1||CIP2||KAP1||FLJ25787||MGC70625||CDKN3.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The CDKN3 solution (0.5 mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 40% glycerol and 0.1M NaCl.

Storage Conditions

CDKN3 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MKPPSSIQTS EFDSSDEEPI EDEQTPIHIS WLSLSRVNCS QFLGLCALPG CKFKDVRRNV QKDTEELKSC GIQDIFVFCT RGELSKYRVP NLLDLYQQCG IITHHHPIAD GGTPDIASCC EIMEELTTCL KNYRKTLIHC YGGLGRSCLV AACLLLYLSD TISPEQAIDS LRDLRGSGAI QTIKQYNYLH EFRDKLAAHL SSRDSQSRSV SR.