CDK1 Recombinant Protein

Source : Sf9, Baculovirus cells. CDK1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (1-297a.a.) and having a molecular mass of 35.1kDa. CDK1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control of the eukaryotic cell cycle through modulating the centrosome cycle in addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1 progress and G1-S transition using association with multiple interphase cyclins. CDK1 is essential in higher cells for the entry into S-phase and mitosis.

Product Specifications

Product Name Alternative

Cyclin-Dependent Kinase 1||CDC2||Cell Division Cycle 2, G1 To S And G2 To M||Cell Division Control Protein 2 Homolog||Cell Division Protein Kinase 1||P34 Protein Kinase||P34CDC2||CDC28A||Cell Cycle Controller CDC2||EC 2.7.11.22||EC 2.7.11.23||CDKN1||

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

CDK1 protein solution (0. 5mg/mL) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIREISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCHSRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSARYSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPEVESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEHHHHHH.