UCHL3 Recombinant Protein
Source : Escherichia Coli. UCHL3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-230a.a.) and having a molecular mass of 28.3kDa.UCHL3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1.
Product Specifications
Product Name Alternative
Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase) ||UCH-L3||Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3||EC 3.4.19.12.
Purification
Greater than 95% as determined by SDS-PAGE.
Components
The UCHL3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT and 10% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Applications Notes
Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.
Amino Acids
Frequently Asked Questions
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