UBE2I Recombinant Protein

Source : Escherichia Coli. Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids & having a molecular mass of 17.9 kDa. Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s) . However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1 (SUMO-1) . And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.

Product Specifications

Product Name Alternative

SUMO-conjugating enzyme UBC9||EC 6.3.2.-||SUMO-protein ligase||Ubiquitin-conjugating enzyme E2 I||Ubiquitin-protein ligase I||Ubiquitin carrier protein I||Ubiquitin carrier protein 9||p18||UBC9||C358B7.1.

Purification

Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The protein (1 mg/mL) contains 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT, and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS.