TaqDNA Recombinant Protein
Source : Recombinant e.coli contains Thermus aquaticus polymerase gene. Taq DNA Polymerase (a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ́~3 ́ direction in the presence of magnesium and also possesses a 5 ́~3 ́ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
Product Specifications
Product Name Alternative
DNA polymerase I thermostable||EC 2.7.7.7||Taq polymerase 1.
Purification
Greater than 95.0% as determined by SDS-PAGE.
Components
Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
Storage Conditions
Stable for 5 days at 10°C, for longer period of time store at -20°C.
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