SPINK1 Recombinant Protein

Source : Escherichia Coli. SPINK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 79 amino acids (24-79) and having a molecular mass of 8.6kDa.SPINK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Trypsin inhibitor SPINK1 is secreted from pancreatic acinar cells into pancreatic fluid. SPINK1 has a role in inhibition of trypsin-catalyzed premature initiation of zymogens in the pancreas and the pancreatic duct. Alterations in SPINK1 gene are the cause for tropical calcific and pancreatitis hereditary pancreatitis.

Product Specifications

Product Name Alternative

Serine Peptidase Inhibitor Kazal Type 1||Pancreatic Secretory Trypsin Inhibitor||Tumor-Associated Trypsin Inhibitor||PCTT||TATI||Spink3||Serine Protease Inhibitor Kazal Type 1||TCP||PSTI.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The SPINK1 solution (0.5mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.