PSMB9 Recombinant Protein

Source : Escherichia Coli. PSMB9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (21-219 a.a.) and having a molecular mass of 23.5kDa.PSMB9 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Proteasome subunit beta type-9 (PSMB9) belongs to the proteasome B-type family which is a 20S core beta subunit. PSMB9 is positioned in the class II region of the MHC (major histocompatibility complex) . Expression of the PSMB9 protein is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome.

Product Specifications

Product Name Alternative

Proteasome subunit beta type-9||Low molecular mass protein 2||Macropain chain 7||Multicatalytic endopeptidase complex chain 7||Proteasome chain 7||Proteasome subunit beta-1i||Really interesting new gene 12 protein||PSMB9||LMP2||PSMB6i||RING12||beta1i

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

PSMB9 solution (1mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 50mM NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MTTIMAVEFD GGVVMGSDSR VSAGEAVVNR VFDKLSPLHE RIYCALSGS AADAQAVADMA AYQLELHGIE LEEPPLVLAA ANVVRNISYK YREDLSAHLM VAGWDQREGG QVYGTLGGML TRQPFAIGGS GSTFIYGYVD AAYKPGMSPE ECRRFTTDAI ALAMSRDGSS GGVIYLVTIT AAGVDHRVIL GNELPKFYDE.