PSMB5 Recombinant Protein

Source : Escherichia Coli. PSMB5 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 241 amino acids (60-263 a.a.) and having a molecular mass of 26.7kDa. The PSMB5 is purified by proprietary chromatographic techniques. PSMB5 belongs to the proteasome B-type family that has a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is substituted by catalytic subunit 3i (proteasome beta 8 subunit) . A fundamental function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB5 exhibits an ATP dependent proteolytic activity and is involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.

Product Specifications

Product Name Alternative

Proteasome subunit beta type-5||Macropain epsilon chain||Multicatalytic endopeptidase complex epsilon chain||Proteasome chain 6||Proteasome epsilon chain||Proteasome subunit MB1||Proteasome subunit X||PSMB5||LMPX||MB1||X||MGC104214.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

The PSMB5 solution (0.25 mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P.