PPP4C Recombinant Protein

Source : E.coli. PP4C Human Recombinant produced in E. coli is a single polypeptidechain containing 330 amino acids (1-307) and having a molecular mass of 37.5kDa. PPP4C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Protein Phosphatase 4 Catalytic subunit (PPP4C), is a part of the Serine/threonine-protein phosphatase catalytic subunits which tskes part in dephosphorylation and regulation of HDAC3. The protein phosphatase (PP) holoenzyme is a trimeric complex compound of a regulatory subunit, a variable subunit and a catalytic subunit. 4 major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C.

Product Specifications

Product Name Alternative

Serine/threonine-protein phosphatase 4 catalytic subunit, PP4||PP4C||PPH3||PPP4||PPX||PPP4C||Protein Phosphatase 4 Catalytic subunit||Protein phosphatase X||PP-X||Pp4.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The PPP4C solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDSPVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHESRQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVPHDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVWSAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL.