PPM1G Recombinant Protein

Source : Escherichia Coli. PPM1G Human Recombinant fused with His-tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 27 kDa.The PPM1G is purified by proprietary chromatographic techniques. PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.

Product Specifications

Product Name Alternative

Protein Phosphatase 1G||PP2CG||PPP2CG||MGC1675||MGC2870||PP2C GAMMA||EC 3.1.3.16||Protein phosphatase 2C isoform gamma||PP2C-gamma||Protein phosphatase magnesium-dependent 1 gamma||Protein phosphatase 1C||PPM1G||PPM1C.

Purification

Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The PPM1G solution (1mg/mL) contains 25mM Tris pH-7.5, 1mM DTT, 1mM EDTA, 2mM b-ME and 20% glycerol.

Storage Conditions

PPM1G although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD.