PPCDC Recombinant Protein
Source : Escherichia Coli. PPCDC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204a.a.) and having a molecular mass of 24.6kDa.PPCDC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. PPCDC is a member of the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily which takes a part in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B) . The biosynthesis of CoA from pantothenate needs several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4''-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC is a key player in this pathway. PPCDC converts 4''-phosphopantothenate into 4''-phosphopantetheine.
Product Specifications
Product Name Alternative
FLJ14585||PPC-DC||MDS018||COAC||CoaC||Phosphopantothenoylcysteine Decarboxylase.
Purification
Greater than 95% as determined by SDS-PAGE.
Components
The PPCDC protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.
Amino Acids
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