NUDT2 Recombinant Protein

Source : Escherichia Coli. NUDT2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-147a.a.) and having a molecular mass of 19.0kDa.NUDT2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NUDT2 belongs to the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. NUDT2 conserves homeostasis by using water to cleave the metabolite NUDT symmetrically back into its original ATP and AMP molecules. In addition, NUDT2 is active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, however, not towards diadenosine triphosphate. NUDT2 has a role in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations.

Product Specifications

Product Name Alternative

Nudix (nucleoside diphosphate linked moiety X) -type motif 2||APAH1||Diadenosine 5',5'''-P1, P4-tetraphosphate asymmetrical hydrolase||Diadenosine tetraphosphatase||Nucleoside diphosphate-linked moiety X motif 2||Ap4Aase||EC 3.6.1.17||MGC10404

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The NUDT2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 0.1M NaCl and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MALRACGLII FRRCLIPKVD NNAIEFLLLQ ASDGIHHWTP PKGHVEPGED DLETALRETQ EEAGIEAGQL TIIEGFKREL NYVARNKPKT VIYWLAEVKD YDVEIRLSHE HQAYRWLGLE EACQLAQFKE MKAALQEGHQ FLCSIEA