NT5M Recombinant Protein

Source : Escherichia Coli. NT5M Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 a.a.) and having a molecular mass of 25.1kDa.NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NT5M is a member of the 5' (3') -deoxyribonucleotidase family. NT5M localizes to the mitochondrial matrix. The NT5M enzyme dephosphorylates the 5'- and 2' (3') -phosphates of uracil and thymine deoxyribonucleotides. The NT5M gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unidentified etiology.

Product Specifications

Product Name Alternative

5' (3') -deoxyribonucleotidase mitochondrial||5',3'-nucleotidase||mitochondrial||Deoxy-5'-nucleotidase 2||dNT-2||NT5M||DNT2.

Purification

Greater than 95% as determined by SDS-PAGE.

Components

NT5M protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC.