NT5C3B Recombinant Protein

Source : Escherichia Coli. NT5C3B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (1-300 a.a) and having a molecular mass of 36.8kDa. NT5C3B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. 7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) includes transferase activity and nucleotide binding. Among NT5C3B's related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. NT5C3B hydrolyzes 7-methylguanosine monophosphate (m (7) GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m (7) GMP guards cells against undesired retrieval of m (7) GMP and its incorporation into nucleic acids. In addition, NT5C3B has weak activity for CMP.

Product Specifications

Product Name Alternative

NT5C3L||7-methylguanosine phosphate-specific 5'-nucleotidase||Cytosolic 5'-nucleotidase 3B||Cytosolic 5'-nucleotidase III-like protein||cN-III-like protein||N (7) -methylguanylate 5'-phosphatase.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

NT5C3B protein solution (1mg/mL) containing Phosphate Buffered Saline (pH7.4), 20% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLSRFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQKIQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFNEDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKVEERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP.