NFNB Recombinant Protein

Source : Escherichia Coli. NFNB Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-217 a.a.) and having a molecular mass of 26 kDa. The NFNB is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. NFNB demonstrates the capability to reduce quinines. NFNB enzyme activates prodrugs in antibody directed enzyme prodrug therapy. NFNB reduces nitrofurazone, quinones and anti-tumor agent CB1954 (5- (aziridin-1-yl) -2,4-dinitrobenzamide) . The reduction of CB1954 results in the generation of cytotoxic species.

Product Specifications

Product Name Alternative

DPRA||NFSB||NFSI||NTR.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

1mg/mL solution containing 20mM Tris pH-8, 1mM DTT, 0.05M NaCl & 10% glycerol.

Storage Conditions

NFNB Human although stable at 4C for 1 week, should be stored desiccated below -18C. Please prevent freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV.