NDUFV3 Recombinant Protein

Source : Escherichia Coli. NDUFV3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 97 amino acids (35-108) and having a molecular mass of 10.8kDa.NDUFV3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NDUFV3 is one of no less than 41 subunits which form the NADH-ubiquinone oxidoreductase complex that is involved in the mitochondrial respiratory chain and assists in catalyzing the rotenone-sensitive oxidation of NADH and the reduction of ubiquinone. NDUFV3 is 1 of 3 proteins found in the flavoprotein fraction of the complex but it exact function is unknown. Two transcript variants encoding altered isoforms are known for this gene.

Product Specifications

Product Name Alternative

NADH Dehydrogenase (Ubiquinone) Flavoprotein 3 10kDa||Renal Carcinoma Antigen NY-REN-4||NADH-Ubiquinone Oxidoreductase 9 KDa Subunit||EC 1.6.5.311||CI-10k||Complex I 10kDa

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

The NDUFV3 solution (0.25mg/mL) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT, 2mM EDTA and 50% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.