NDUFB4 Recombinant Protein

Source : E.Coli NDUFB4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 110 amino acids (1-87) and having a molecular mass of 12.6kDa.NDUFB4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NADH Dehydrogenase 1 Beta Subcomplex 4 (NDUFB4) is a member of the complex I NDUFB4 subunit family. NDUFB4 is a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, which is the first enzyme complex in the mitochondrial electron transport chain (complex I) . Mammalian complex I is comprised of 45 different subunits and transfers electrons from NADH to ubiquinone.

Product Specifications

Product Name Alternative

B15||CI-B15||Complex I-B15||NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4||NADH-ubiquinone oxidoreductase B15 subunit.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

The NDUFB4 solution (1mg/mL) contains 20mM Tris-HCl buffer, (pH 8.0), 0.4M Urea And 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.