NDUFAF4 Recombinant Protein

Source : Escherichia Coli. NDUFAF4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-175) and having a molecular mass of 22.7kDa.NDUFAF4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 (NDUFAF4) is involved in the compilation of mitochondrial NADH: ubiquinone oxidoreductase complex (complex I) . In addition, NDUFAF4 is involved in cell proliferation and survival of hormone-dependent tumor cells. NDUFAF4 may also be a regulator of breast tumor cell invasion. NDUFAF4 gene mutations cause the mitochondrial complex I deficiency.

Product Specifications

Product Name Alternative

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4||Hormone-regulated proliferation-associated protein of 20 kDa||NDUFAF4||C6orf66||HRPAP20||HSPC125||My013||bA22L21.1.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

The NDUFAF4 solution (0.5mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMGALVIR GIRNFNLENR AEREISKMKP SVAPRHPSTN SLLREQISLY PEVKGEIARK DEKLLSFLKD VYVDSKDPVS SLQVKAAETC QEPKEFRLPK DHHFDMINIK SIPKGKISIV EALTLLNNHK LFPETWTAEK IMQEYQLEQK DVNSLLKYFV TFEVEIFPPE DKKAIRSK.